WebGiven the severity of the COVID-19 outbreak, Chroma's top priority remains the health and well-being of our employees, customers and greater communities. WebRESEARCH ARTICLE Improved 3D cellular morphometry of Caenorhabditis elegans embryos using a refractive index matching medium Rain Xiong1,2, Kenji Sugioka ID 1,2* 1 Life Sciences Institute, The University of British Columbia, Vancouver, BC, Canada, 2 Department of Zoology, The University of British Columbia, Vancouver, BC, Canada
TagRFP Antibody (R10367) - Thermo Fisher Scientific
WebMay 31, 2012 · Tandem fluorescent-tagged LC3 (mRFP-EGFP-LC3) is a convenient assay for monitoring autophagic flux based on different pH stability of EGFP and mRFP fluorescent proteins. However, it has been … WebFor example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors: These FPs change their excitation/emission behavior upon environmental changes (e.g. pH, Ca 2+ flux, etc). The most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato (pH sensors), HyPer (H 2 O 2 sensor ... suffer hat
Optical probing of a dynamic membrane interaction that …
WebNov 13, 2012 · The mutations K69R and R203H in the region surrounding the chromophore from a close homologue, TagRFP (Supplementary Fig. S1), were transferred to mKate2.5 and resulted in dramatically enhanced pH ... Webcell coexpressing tagRFP-PH PLC and EGFP-PH OSH1 (Fig. 1C). We extended this technique to see whether we can track changes in phospholipids induced by activation of G protein coupled receptors (GPCRs). We coexpressed EGFP-PH PLC with the 5HT2c receptor, a GPCR coupled to the Gq α-subunit of G protein (i.e., a GqPCR), whose receptor activation ... WebAug 19, 2016 · The recombinant TagRFP-T protein was eluted with a linear 0–0.5 M NaCl gradient by automatically mixing anion exchange buffer A and buffer B (20 mM Tris-HCl, pH 8.0, 1 M NaCl and 10% glycerol). Protein fractions eluted throughout the purification process were analyzed by SDS-PAGE. sufferhead