WebDec 24, 2015 · The promoter of the homologous thl gene (Pthl) cloned from C. tyrobutyricum, the fdh gene and pMTL-adhE2 backbone were assembled to generate the plasmid pMTL-fdh-adhE2 using the Gibson Assembly kit (NEB) following the manufacture’s instruction. Figure 2. Plasmid construction. WebJun 12, 2024 · Using GusA, a library of synthetic promoters was first generated by a typical approach entailing complete randomization of a constitutive thiolase gene promoter (Pthl) except for the consensus -35 ...
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WebJun 9, 2024 · To probe the functional roles of the four different SL structures , a dual-fluorescence reporter system was constructed using the Ccel–Ecoli shuttle vector pMTC6, which harbors two reporter genes: (i) fbfp (encoding green fluorescence protein) coupled with the pthl promoter (Cui et al., 2012) and (ii) mCherry (encoding red fluorescence ... WebPlasmid pIMP1-Pthl from Dr. Sheng Yang's lab is published in J Microbiol Methods. 2015 Jan;108:49-60. doi: 10.1016/j.mimet.2014.11.004. Epub 2014 Nov 15. ... (Empty … how many times paul was imprisoned
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WebOct 18, 2016 · Design and screening of tetracycline-inducible promoter variants with catP as a reporter. Shown in a are the sequences of the original Tet3n0 promoter and seven synthetic inducible promoters. The randomized non-conserved bases in −35 and −10 promoter elements are highlighted in red.Shown in b is the activity of catP reporter under … WebThe protein encoded by this gene is a member of the parathyroid hormone family. This hormone, via its receptor, PTHR1, regulates endochondral bone development and … WebThis composite part is composed of the Pcrt promoter (BBa_K4515009), codon-optimized crt gene (BBa_K4515006), hbd gene (BBa_K4515005), ter gene (BBa_K4515007), Pthl promoter (BBa_K4515008), and thl gene (BBa_K4515004) [1-6], and was constructed into the pIB184 vector backbone to construct the pLY15-opt plasmid (Figure 1). Figure 1. The … how many times paul shipwrecked