Pcmv-mcherry-gfp-lc3b
SpletA: 一般做自噬流检测我们不建议细胞再带其他颜色的荧光,例如是做基因敲减,有三种解决方案:. 1)建议将目的基因shRNA构建在自噬载体上(该载体已经带有红绿荧光),那么进行自噬慢病毒感染即可,该方案的优点是只感染一次病毒,操作简便,缺点是重新 ... SpletGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统 自噬形成时,GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜,在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点。 当自噬溶酶体形成后,酸性环境使GFP荧光淬灭,而mCherry荧光不受影响,自噬溶酶体呈现红色荧光。 因此,可以通过LC3荧光指示系统来监测自噬流。 LC3自噬双标系统追踪自噬流 …
Pcmv-mcherry-gfp-lc3b
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SpletPlasmid pCMV-FKBP-mCherry-MCS from Dr. Shinya Tsukiji's lab contains the insert FKBP-mCherry and is published in J Am Chem Soc. 2024 May 5;143(17):6434-6446. doi: … Splet02. jun. 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to …
http://www.labbase.net/Supply/SupplyItems-4229347.html SpletpCMV-GFP-LC3B是碧云天自行研发的在哺乳动物细胞中表达GFP标签的人源LC3B 融合蛋白的质粒。该质粒含有CMV启动子,为卡那霉素抗性,转染细胞后能够在靶细胞中高效表达绿色荧光蛋白GFP标签的LC3B的融合蛋白,呈现明亮的绿色荧光,可以用于细胞自噬(autophagy)的研究。
Splet07. nov. 2015 · Expression fluorescentprotein could TZM-tfLC3Bcells follow- ing more than 50 passages which indicated cellline (data shown).Fig. TZM-tfLC3Bcells expressed LC3B … SpletpCDH-EF1a-mCherry-EGFP-LC3B (Plasmid #170446) Print Enlarge View all sequences Purpose Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To …
SpletPrice:888.00RMB. •. Purpose. Express Human LC3B protein with N-terminal mCherry and GFP tag in mammalian cell. •. Depositor. Beyotime. •. Publication.
SpletThis website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies. ife sugeySpletFUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060 ; http://n2t.net/addgene:110060 ; RRID:Addgene_110060) Lysosome activation clears … is snaptube available for pcSpletList of McHenry County DMV Locations. Woodstock Secretary of State Office 428 Eastwood Drive Woodstock IL 60098 312-793-1010. is snapware dishwasher safeSpletPlasmid pCDH-CMV-mC-G-LC3B-P from Dr. Kazuhiro Oka's lab contains the insert mCherry-GFP-LC3B. This plasmid is available through Addgene. ifes wikipediaSpletLC3Bは、オートファジーの早い段階で細胞質からリクルートされ、隔離膜と結合します。 この局在化は、オートファジー膜およびその過程をモニタリングするための一般的マーカーとしての役割を果たします。 Molecular Probes®のツールにより以下のことが可能です: 生細胞や固定細胞における追跡と局在化 リアルタイムでのLC3B含有小胞のpHモニタ … ife susySpletPremo Autophagy Tandem Sensor RFP-GFP-LC3B Kit: Format: Tube: Kit Contents: Autophagy Sensor RFP-GFP-LC3B, and chloroquine diphosphate: Product Type: … ife subsidioSplet12. maj 2024 · mCherry-GFP-LC3B自噬双标系统的工作原理为: 未发生自噬的细胞及含有自噬体的细胞中,由于mCherry与GFP共同表达,细胞呈现黄色荧光。 当自噬体与溶酶体融合形成自噬溶酶体后,酸性的溶酶体环境使酸敏感的GFP荧光淬灭,而mCherry不受影响,进而使自噬溶酶体呈现 ... is snapware glasslock freezer safe