WebC-Myc 标签蛋白，是一个含11个氨基酸的小标签，这11个氨基酸作为抗原表位表达在不同的蛋白质框架中仍可识别其相应抗体。 C-Myc tag已成功应用在 Western-blot杂交技术、免疫沉淀和流式细胞计量术中, 可用于检测 … Web11 jan. 2005 · MYCN was detected as described in Western blot analysis. ChIP. ChIP cloning was performed with lysates of the JF MYCN -amplified neuroblastoma cell line. Using a modified version of published protocols ( 19, 20 ), we performed two rounds of immunoprecipitation with an anti-MYCN mAb.
BCAT1 Overexpression Promotes Proliferation, Invasion, and …
WebThe c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and … WebWestern blot analysis of c-Myc. Performed by loading 25 µg of 293t cells transfected with c-Myc -MYCBP (lane 1), transfected with c-Myc -FLI1 (lane 2) and transfected with 12Tag (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Myc Tag Polyclonal … thinpro os download
Recombinant Anti-c-Myc antibody [Y69] - ChIP Grade (ab32072)
WebThe Western blot you see on the datasheet represents the blot from a gel wherein 5ug of lysate # NBL1-13414 was loaded. However, to our customers, we generally recommend using 5-10ug of lysate because the antibody that we used in our validation of the lysate might be different from what customers would be using. WebMyc transcription factors are considered as the main oncoproteins responsible for glutamine addiction of tumor cells [22]. c-Myc drives glutamine uptake and catabolism by activating the expression of genes involved in glutamine metabolism, including glutaminase, and … WebFor western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower … thinprint server