WebA physical map of the genome of Rhizobium meliloti 1021 is presented. The physical sizes of the three replicons in this genome had previously been determined and are as follows: the chromosome, 3.4 Mb; pSym-b, 1.7 Mb; and pSym-a, 1.4 Mb. The physical maps for this GC-rich genome contain AT-rich rest … WebA combined physical and genetic map of theCorynebacterium glutamicum ATCC 13032 chromosome was constructed using pulsed-field gel electrophoresis (PFGE) and hybridizations with cloned gene probes. Total genomic DNA was digested with the meganucleasesSwaI (5′-ATTTAAAT-3′),PacI (5′-TTAATTAA-3′), andPmeI (5′ …
Electroporation of Pichia pastoris SpringerLink
WebPmeI (GTTTAAAC) site and the deletion of the SpeI from the origi-nal vector. Construction of replicon pBAC-SARS-CoV-DEDS/eGFP. The previ-ously constructed infectious cDNA clone (plasmid rSARS-CoV-DE) [25] was digested with PmeI and BamHI producing a 7.5 kb frag-ment containing 30 end of pp1b, S and 3a gene. This fragment WebVitellogenesis, the timely production and uptake of yolk proteins, is essential for female fecundity. In the silkworm, Bombyx mori, a nucleus‐enriched long‐noncoding RNA (lncRNA) lncR26319 regulates Endophilin A (EndoA), a member of the endophilin protein family of endocytosis, by competitively binding to miR‐2834.The lncR26319‐miR‐2834‐EndoA … theorie fragen a1
Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and Their ...
WebJul 15, 2013 · To obtain a plasmid, containing the cDNA of a packaging construct for ChikRepl-Gluc, the region of the infectious cDNA corresponding to nucleotides 308–7419 of CHIKV-LR2006 OPY1 was replaced by sequence 5’ GTTTAAAC 3’ (recognition site of Mss I restriction enzyme) using PCR-mediated mutagenesis; the resulting plasmid was … WebSep 13, 1996 · Total genomic DNA was digested with the meganucleases SwaI (5'-ATTTAAAT-3'), PacI (5'-TTAATTAA-3'), and PmeI (5'-GTTTAAAC-3') yielding 26,27, and 23 fragments, respectively. The chromosomal restriction fragments were then separated by PFGE. By summing up the lengths of the fragments generated with each of the three … WebTECHNICAL REFERENCE PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD MADISON, WI 53711-5399 USA TELEPHONE 608-274-4330 www.promega.com ©2010 ALL RIGHTS RESERVED PART #GE646 Site-Specific Methylation Sensitivity of … theoriefragen klasse b online