DIPEA is a sterically hindered organic base that is commonly employed as a proton scavenger. Thus, like 2,2,6,6-tetramethylpiperidine and triethylamine, DIPEA is a good base but a poor nucleophile, DIPEA has low solubility in water, which makes it very easily recovered in commercial processes, a combination of properties that makes it a useful organic reagent. WebApr 10, 2024 · To a solution of intermediate 49 (200 mg, 0.63 mmol) in t-BuOH (5 mL) was added aniline (176.1 mg, 1.26 mmol), DIPEA (126.8 mg, 1.26 mmol). After being stirred at 100 °C for 12 h in a sealed tube, the reaction mixture was cooled to rt, diluted with H 2 O and extracted thrice with ethyl acetate.
DIPEA Sigma-Aldrich
WebMar 16, 2024 · N,N-dimethylformamide (DMF), CH 2 Cl 2, MeCN, MeOH, Et 2 O, AcOH, Ac 2 O, N,N-diisopropylethylamine (DIPEA), trifluoroacetic acid ... Gibco), 100 U/ml penicillin-streptomycin (Thermo Fisher Scientific) and 2 mg/ml puromycin (Thermo Fisher Scientific) at 37 °C under 5% CO 2. For each assay, 40,000 cells per well and 100 μl per well, were ... WebDIPEA Ethyldiisopropylamine Formula : C 8 H 19 N . Sigma-Aldrich - 550043 Page 3 of 11 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada Molecular weight : 129.24 g/mol CAS-No. : … mellon net worth
Synthesis and Purification of Peptide Nucleic Acids
WebAug 29, 2013 · To synthesize the biotin-containing probe, make a solution of biotin (74 mg, 300 μmol), HBTU (114 mg, 300 μmol) and DIPEA (104 μl, 600 μmol) in NMP (7 ml). Add the solution to the resin and ... WebActive esters generated with most commonly used coupling reagents. The most commonly employed reagents, BOP, PyBOP, and HBTU generate OBt esters, and these have found wide application in routine SPPS and … WebApplied Biosystems Thermo Fisher Scientific - US Applied Biosystems Where genomics comes together. From the gold-standard technology that helped power the Human Genome Project to the in-house expertise that has continued to support genomic breakthroughs the world over, Applied Biosystems solutions are where genomics … mellon national bank and trust company